Growth Intensity of Trichoderma Viride at Different Doses and Sources of Copper in the Medium

Abstract

The biological properties of Trichoderma Viride fungi allow them to be effectively used in the technologies of crop waste disposal, production of organic, environmentally friendly fertilisers, and prevention of soil diseases of plants. The growth and development of these fungi depend on a number of trace elements that are found in the medium. Copper is of great importance among trace elements. The establishment of the ability to use copper as a stimulator of biomass accumulation of Trichoderma Viride fungi is of scientific and practical interest for national economic needs, which indicates the relevance of the study. The purpose of the study is to establish the effectiveness of the effect of various copper compounds on the growth and development of Trichoderma Viride. To investigate the effect of the metal-biotic on the growth of microorganisms, 0.5 to 10.0 mg/100 cm3 of the element in the form of copper sulphate, mixed ligand complex and copper glycinate were added to the medium with potato dextrose agar (PDA). In the control variant, the element was not added to the nutrient medium. The media were inoculated with the Viridin preparation. Trichoderma Viride growth was recorded on days 4 and 7 of the experiment. It has been experimentally established that the fungal population is affected by the content and source of copper in the medium. It was found that the presence of a biotic metal in the medium in mineral or chelated form is up to 1.0 mg/100 cm3 stimulates the build-up of fungal biomass. It was proved that on the 4th day of cultivation for the introduction of copper into the medium in the amount of 1.0 mg/100 cm3 in the mixed-ligand form, the population of Trichoderma Viride increased by 75.0%. On the 7th day, the increase in the number of fungal cells was 58.3%. It was found that with the introduction in the medium of more than 5.0 mg/100 cm3 copper in any form, the population growth of Trichoderma Viride fungi decreased. The higher the copper content in the medium, the greater the inhibition of fungal cell reproduction. It was generalised that comparing the action of the mixed ligand complex copper, copper glycinate, and copper sulphate, it was found that the latter compound is the most toxic against Trichoderma Viride.