Вплив аквахелатного розчину Селену на динаміку вмісту імуноглобулінів та циркулюючих імунних вмісту імуноглобулінів та циркулюючих імунних
Вантажиться...
Дата
Назва журналу
Номер ISSN
Назва тому
Видавець
Анотація
В статті показано, що сучасні методи ведення птахівництва передбачають інтенсивні технології вирощування
перепелів, в результаті яких збільшується кількість стрес-факторів, що може бути причиною зниження резистентно-
сті птиці і як наслідок розвиток імунодефіцитного стану. Висвітлено результати наукових досліджень із питань ви-
користання наноаквахелатного розчину Селену та його вплив на показники гуморального імунітету перепелів, зок-
рема, вмісту імуноглобулінів та циркулюючих імунних комплексів в сироватці крові. Встановлено, що аквахелатний
розчин Селену, за інкубаційної обробки перепелиних яєць, впливає на показники гуморального імунітету залежно
від дози. В оптимальній дозі 0,05 мкг/кг аквахелатний розчин Селену сприяє збільшенню вмісту імуноглобулінів та
зменшенню циркулюючих імунних комплексів в сироватці крові перепелів в одно- та п’ятидобовому віці.В статье показано, что современные методы ведения птицеводства предусматривают интенсивные технологии
выращивания перепелов, в результате которых увеличивается количество стресс-факторов, что может быть причи-
ной снижения резистентности птицы и как следствие развитие иммунодефицита. Представлены результаты научных исследований по вопросам использования наноаквахелатного раствора Селена и его влияние на показатели гумора-
льного иммунитета перепелов, в частности, содержания иммуноглобулинов и циркулирующих иммунных комплек-
сов в сыворотке крови. Установлено, что аквахелатный раствор Селена, при инкубационной обработке перепелиных
яиц, влияет на показатели гуморального иммунитета в зависимости от дозы. В оптимальной дозе 0,05 мкг/кг аквахе-
латний раствор Селена способствует увеличению содержания иммуноглобулинов и уменьшению циркулирующих
иммунных комплексов в сыворотке крови перепелов в одно- и пятисуточном возрасте.
Опис
Nowadays, as in humane and veterinary medicine, actively conducted search tools to improve the overall resistance of
the organism and antioxidants. Selenium is part of a series of enzymes that together with catalase and superoxide dismutase
make enzymatic antioxidant defense system of the body. Functional activity selenium containing enzymes (glutathione
peroxidase, peroxidase selenium depended neutrophils, selenium protein, tioredoksynreduktaz et al.) It is ensured by features
of the crystal structure of selenium, which is their focal point.
As selenium immunomodulator regulates the synthesis of antibodies, increases the activity of lymphocytes. Selenium
improves the ability of lymphocytes respond to stimulation of antigen, proliferate and differentiate. Special feature of its
actions is that it activates factors of natural resistance: cell monocyte-macrophage system, NG and NK-cells. Activation of
macrophages leads to increased synthesis of almost all cytokines produced by these cells, because that is enhanced functional
activity of factors both cellular and humoral immunity. That is, under the influence of selenium changes occur throughout the
immune system, which corresponds to the natural course of immune activation observed and the development of any immune
response.
Found that the content of total serum immunoglobulin in the first group as the first and the fifth day tended to increase
compared with the control group, indicating a negligible impact akvahelatae solution of Selenium in the studied dose of
immunoglobulin synthesis.
A second group of quail 1-day age was 7.30±0.07 mg/ml on the 5th day 8.40±0.10 mg/ml, which is significantly
more than the control at 10.6 % (p<0.05) and 7.7 % (p<0.01), an increase of total immunoglobulin is probably a
consequence of activating action chelate solution of selenium in quail specific immunoreactivity body during early
postnatal development. However, in the third group of indicators of total IgG in serum quail had the opposite trend, that
has undergone reduction.
Content Ig M in serum quail, which is the body responsible for the primary immune response, in the first experimental
group in day age tended to increase compared with the control, but at the 5th day their contents probably was higher
compared with the control group at 7.8 % (p<0.05). In group 2 content Ig M in serum was significantly increased in quail day
age of 8.0 % (p<0.05), and four days increased by 23.6 % (p<0.01) compared with control group. This could indicate that
quail increase body resistance due to stimulation of the primary immune response. However, in the 3rd experimental group,
the quail 1-day old content Ig M was 0.22±0.01 mg/ml, which was significantly less than 12 % (p<0.01) than in the control
and five days age only marked downward trend in this indicator.
We know that in the body after the synthesis of Ig M reached a higher stage of the immune response-formation Ig
G. Thus, their content in the blood serum of quails 1-and 5 days old in the first group was significantly more than 2.9 % and
2.7 % compared to control (p<0.05). In group 2 content Ig G in serum quail days age was 5.69±0.04 mg/ml, and after four
days of 6.78±0.03 mg/ml, 3.6 % (p<0.05) and 3.7 % (p<0.01), respectively, more than in the control group. This indicates
that under the influence of akvahelatae solution of selenium probably already happened body increase resistance of quail in
the critical phase of quail. However, in the third group watched a tendency to reduction of Ig G in blood serum as a single
poultry and five days age.The content of Ig A serum in the first group of quail quail 1- and 5 days old tended to increase relative to controls. This
likely change, we have recorded in the 2nd experimental group where Ig and content was 0.73±0.02 mg/ml, which is
1.2 times more compared to control (p<0.05). And on the fifth day content Ig A serum poultry increased compared with the
control group 1.1 times (p<0.01). This indicates that akvahelatae solution of selenium in small doses promotes immune
defense of the organism quail. However, in the 3rd group observed the opposite in the direction of reduction of Ig A serum
quail aged 1–5 days.
It is known that the formation of immune complexes as physiological products antigen-antibody reaction is part of the
defense mechanisms, that is one of the components of the immune response. However, their continued circulation in the body
leads to accumulation of the latter in the tissues, increased platelet aggregation, which in turn, causes abnormal blood
microcirculation.
In the 1- and 5 day age quail in serum in the first experimental group significant difference medium size and low CIC
compared with the control group was not established. However, in the group 2 days old quails in serum showed a significant
decrease in CIC average size of 1.4 % and the small size of 1.1 % (p<0.05), while the heel day observed only a tendency to
decrease these indicators Reduction of serum CIC research groups points to reduce the formation of it antigens and increase
the reactivity of the immune system to their elimination. However, in the 3rd experimental group content of circulating
immune complexes in the blood serum of quails tended to increase in day age, and on the fifth day was greater than the
content of low CIC 6.9 % (p<0.05) compared with control group.
Бібліографічний опис
Ніщеменко М.П. Вплив аквахелатного розчину селену на динаміку вмісту імуноглобулінів та циркулюючих імунних вмісту імуноглобулінів та циркулюючих імунних / М.П. Ніщеменко, О.В. Ємельяненко, А.А. Ємельяненко // Наук. вісник вет. медицини: зб-к наук. праць. - Біла Церква: БНАУ, 2016. - Вип. 1 (127). - С.116-120.