Удосконалення елементiв технологiї мiкроклонального розмноження Cornus mas L.

Abstract

There is a number of problems with the reproduction and spread of cornel – extremely valuable fruit crop in Ukraine. A promising way of cornel reproduction is the use of in vitro microclonal propagation. Through a number of experiments, we have established a number of technological techniques, that can improve the process of microclonal propagation of Cornus mas L. (varieties Nizhnyi and Exotic) can be improved of at the stage of introduction into an aseptic culture by the following technological methods: 1) the explants selection in the phase of "green cone"; 2) decontamination with Blanidas 300 preparation (7 g/l of autoclave distillate); 3) selection of explants from the buds of the medial part of the shoots; 4) cultivation of donor plants under the conditions of the depositary; 5) combined use of ascorbic acid antioxidants (15 mg/l) and polyvinylpyrrolidone (0.5 g/l) through their adding to the nutrient medium