Effect of Roasted Soybean and Canola Seeds on Peroxisome Proliferator‐Activated Receptors Gamma (PPARG) Gene Expression and Cattle Milk Characteristics

Abstract

The aim of this research was to investigate the changes in milk yield and its composition, fatty acid profile and peroxisome proliferator-activated receptors gamma (PPARG) gene expression in adipose tissue of Ira-nian Holstein cattle in response to feeding isonitrogenous and isocaloric diets but formulated based on dif-ferent oilseeds (soybean or canola seeds). Twenty cows were tested randomly with experimental diets. Sampling from subcutaneous adipose tissue was performed. Moreover, fatty acid composition was ana-lyzed, total RNA was isolated, cDNA synthesized and samples were amplified. SPSS 16.0, LinRegPCR and REST software were used to analyze real-time polymerase chain reaction results of gene expression. The amount of PPARG gene expression in the adipose tissue for canola seed treatment, in comparison with the soybean seed was higher. The milk production, 4% fat corrected milk, fat percentage, milk urea nitrogen and body condition score between two treatments was significant (P<0.05).