The separation and detection of several mycotoxins by thin-layer chromatography

Abstract

The article described metods alternatively, T-2 toxin will afford a bright blue fluorescence if treated with a mixture of aluminium chloride (in water:ethanol, 1:1) and chromotropic acid (in concentrated sulphuric acid:water, 5:3) followed by heating at 11 0 °C. The natural fluorescence of sterigmatocystin may be enhanced, to afford a bright yellow spot, by spraying with a 24 percent solution of aluminium chloride in 95 percent aqueous ethanol and heating at 105 °C for 10 minutes. The identity of sterigmatocystin may be confirmed by the formation of the acetate or hemiacetal derivatives. Similarly, the long-wave fluorescence of zearalenone and ochratoxin A may be enhanced if the plate is sprayed with aluminium chloride solution. The presence of ochratoxin A may be confirmed by the formation of the ethyl ester derivative.